I have been having some issues trying to get my first v9 site live on a production server with IIS. Locally it worked ok, once I set the App Pool Advanced Settings "Enable 32bit Applications" to true. If I leave it at false, both locally and on production, I get

The app was likely published for a different bitness than w3wp.exe/iisexpress.exe is running as.

Not sure that is relevant but just in case...

Anyway now I'm getting a 500 error "currently unable to handle this request."

This is the Log file, sorry it's so long but I thought it best to show it all

{"@t":"2022-03-31T11:20:15.1839398Z","@mt":"Acquiring MainDom.","SourceContext":"Umbraco.Cms.Core.Runtime.MainDom","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.1849118Z","@mt":"Acquired MainDom.","SourceContext":"Umbraco.Cms.Core.Runtime.MainDom","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.4654756Z","@mt":"Profiler is VoidProfiler, not profiling (must run debug mode to profile).","SourceContext":"Umbraco.Cms.Web.Common.Profiler.InitializeWebProfiling","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.5099825Z","@mt":"No XML encryptor configured. Key {KeyId:B} may be persisted to storage in unencrypted form.","@r":["{bc73e41e-a446-422b-b0a6-7716e6dee1d8}"],"@l":"Warning","KeyId":"bc73e41e-a446-422b-b0a6-7716e6dee1d8","EventId":{"Id":35,"Name":"NoXMLEncryptorConfiguredKeyMayBePersistedToStorageInUnencryptedForm"},"SourceContext":"Microsoft.AspNetCore.DataProtection.KeyManagement.XmlKeyManager","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"WARN "}
{"@t":"2022-03-31T11:20:15.8106161Z","@mt":"Application started. Press Ctrl+C to shut down.","SourceContext":"Microsoft.Hosting.Lifetime","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.8106953Z","@mt":"Hosting environment: {envName}","envName":"Production","SourceContext":"Microsoft.Hosting.Lifetime","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.8107126Z","@mt":"Content root path: {contentRoot}","contentRoot":"D:\\inetpub\\Website\\www","SourceContext":"Microsoft.Hosting.Lifetime","ProcessId":4572,"ProcessName":"w3wp","ThreadId":1,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"INFO "}
{"@t":"2022-03-31T11:20:15.9688959Z","@mt":"Connection ID \"{ConnectionId}\", Request ID \"{TraceIdentifier}\": An unhandled exception was thrown by the application.","@l":"Error","@x":"System.UnauthorizedAccessException: Access to the path 'D:\\inetpub\\Website\\www\\umbraco\\Data\\TEMP\\NuCache\\NuCache.Content.db' is denied.\r\n   at System.IO.FileStream.ValidateFileHandle(SafeFileHandle fileHandle)\r\n   at System.IO.FileStream.CreateFileOpenHandle(FileMode mode, FileShare share, FileOptions options)\r\n   at System.IO.FileStream..ctor(String path, FileMode mode, FileAccess access, FileShare share, Int32 bufferSize, FileOptions options)\r\n   at CSharpTest.Net.IO.TransactedCompoundFile..ctor(Options options)\r\n   at CSharpTest.Net.Storage.BTreeFileStoreV2..ctor(Options options)\r\n   at CSharpTest.Net.Collections.BPlusTree`2.OptionsV2.CreateStorage()\r\n   at CSharpTest.Net.Collections.BPlusTree`2.NodeCacheBase..ctor(BPlusTreeOptions`2 options)\r\n   at CSharpTest.Net.Collections.BPlusTree`2.NodeCacheNone..ctor(BPlusTreeOptions`2 options)\r\n   at CSharpTest.Net.Collections.BPlusTree`2..ctor(BPlusTreeOptions`2 ioptions)\r\n   at CSharpTest.Net.Collections.BPlusTree`2..ctor(OptionsV2 optionsV2)\r\n   at Umbraco.Cms.Infrastructure.PublishedCache.DataSource.BTree.GetTree(String filepath, Boolean exists, NuCacheSettings settings, ContentDataSerializer contentDataSerializer)\r\n   at Umbraco.Cms.Infrastructure.PublishedCache.PublishedSnapshotService.MainDomRegister()\r\n   at Umbraco.Cms.Core.Runtime.MainDom.Register(Action install, Action release, Int32 weight)\r\n   at Umbraco.Cms.Infrastructure.PublishedCache.PublishedSnapshotService.<EnsureCaches>b__47_0()\r\n   at System.Threading.LazyInitializer.EnsureInitializedCore[T](T& target, Boolean& initialized, Object& syncLock, Func`1 valueFactory)\r\n   at System.Threading.LazyInitializer.EnsureInitialized[T](T& target, Boolean& initialized, Object& syncLock, Func`1 valueFactory)\r\n   at Umbraco.Cms.Infrastructure.PublishedCache.PublishedSnapshotService.EnsureCaches()\r\n   at Umbraco.Cms.Infrastructure.PublishedCache.PublishedSnapshotService.CreatePublishedSnapshot(String previewToken)\r\n   at Umbraco.Cms.Web.Common.UmbracoContext.UmbracoContext.<>c__DisplayClass11_0.<.ctor>b__0()\r\n   at System.Lazy`1.ViaFactory(LazyThreadSafetyMode mode)\r\n   at System.Lazy`1.ExecutionAndPublication(LazyHelper executionAndPublication, Boolean useDefaultConstructor)\r\n   at System.Lazy`1.CreateValue()\r\n   at System.Lazy`1.get_Value()\r\n   at Umbraco.Cms.Web.Common.UmbracoContext.UmbracoContext.get_PublishedSnapshot()\r\n   at Umbraco.Cms.Web.Common.UmbracoContext.UmbracoContext.get_Content()\r\n   at Umbraco.Cms.Web.Website.Routing.UmbracoRouteValueTransformer.TransformAsync(HttpContext httpContext, RouteValueDictionary values)\r\n   at Microsoft.AspNetCore.Mvc.Routing.DynamicControllerEndpointMatcherPolicy.ApplyAsync(HttpContext httpContext, CandidateSet candidates)\r\n   at Microsoft.AspNetCore.Routing.Matching.DfaMatcher.SelectEndpointWithPoliciesAsync(HttpContext httpContext, IEndpointSelectorPolicy[] policies, CandidateSet candidateSet)\r\n   at Microsoft.AspNetCore.Routing.EndpointRoutingMiddleware.<Invoke>g__AwaitMatch|8_1(EndpointRoutingMiddleware middleware, HttpContext httpContext, Task matchTask)\r\n   at SixLabors.ImageSharp.Web.Middleware.ImageSharpMiddleware.Invoke(HttpContext context)\r\n   at Microsoft.AspNetCore.Diagnostics.StatusCodePagesMiddleware.Invoke(HttpContext context)\r\n   at StackExchange.Profiling.MiniProfilerMiddleware.Invoke(HttpContext context) in C:\\projects\\dotnet\\src\\MiniProfiler.AspNetCore\\MiniProfilerMiddleware.cs:line 119\r\n   at Umbraco.Cms.Web.Common.Middleware.UmbracoRequestMiddleware.InvokeAsync(HttpContext context, RequestDelegate next)\r\n   at Umbraco.Cms.Web.Common.Middleware.UmbracoRequestMiddleware.InvokeAsync(HttpContext context, RequestDelegate next)\r\n   at Microsoft.AspNetCore.Builder.UseMiddlewareExtensions.<>c__DisplayClass6_1.<<UseMiddlewareInterface>b__1>d.MoveNext()\r\n--- End of stack trace from previous location ---\r\n   at Umbraco.Cms.Web.Common.Middleware.PreviewAuthenticationMiddleware.InvokeAsync(HttpContext context, RequestDelegate next)\r\n   at Microsoft.AspNetCore.Builder.UseMiddlewareExtensions.<>c__DisplayClass6_1.<<UseMiddlewareInterface>b__1>d.MoveNext()\r\n--- End of stack trace from previous location ---\r\n   at Umbraco.Cms.Web.Common.Middleware.UmbracoRequestLoggingMiddleware.InvokeAsync(HttpContext context, RequestDelegate next)\r\n   at Microsoft.AspNetCore.Builder.UseMiddlewareExtensions.<>c__DisplayClass6_1.<<UseMiddlewareInterface>b__1>d.MoveNext()\r\n--- End of stack trace from previous location ---\r\n   at Microsoft.AspNetCore.Server.IIS.Core.IISHttpContextOfT`1.ProcessRequestAsync()","ConnectionId":"11096869487746541948","TraceIdentifier":"8000e97d-0001-9a00-b63f-84710c7967bb","EventId":{"Id":2,"Name":"ApplicationError"},"SourceContext":"Microsoft.AspNetCore.Server.IIS.Core.IISHttpServer","RequestId":"8000e97d-0001-9a00-b63f-84710c7967bb","RequestPath":"/","ProcessId":4572,"ProcessName":"w3wp","ThreadId":5,"AppDomainId":1,"AppDomainAppId":"d21e3b97ecadec4f3bba7ed9f628fb1336469877","MachineName":"SQL22","Log4NetLevel":"ERROR"}